Fig. 3: Lower magnitude of antigen-specific memory B cells in the vaccinated SLE cohort. | Nature Immunology

Fig. 3: Lower magnitude of antigen-specific memory B cells in the vaccinated SLE cohort.

From: Disease-associated B cells and immune endotypes shape adaptive immune responses to SARS-CoV-2 mRNA vaccination in human SLE

Fig. 3

a, Cartoon showing the ex vivo tetramer-based detection of spike- and RBD-reactive B cells and high-dimensional flow immunoprofiling of B cells from PBMCs. b, Representative fluorescence-activated cell sorting (FACS) plots showing the gating strategy applied to characterize the total CD19+CD20+ B cells (excluding the CD20−CD38hi plasma cells) binding to dual-tetrameric spike probes and tetrameric RBD probes. c,d, Quantification of the total spike-specific (c) and RBD-specific (d) B cells shown as the frequency of CD20+ B cells in the HD and SLE cohorts. Each dot represents an individual sample tested at baseline (day 0) and after receiving one (Vax1), two (Vax2) or three (Vax3) vaccine doses. Differences among groups were analyzed using multiple-group comparisons by nonparametric Kruskal–Wallis statistical testing using Dunn’s post hoc analysis in GraphPad Prism. Comparisons using pie charts and a chi-square with Fisher’s test are shown. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. S, spike; bio–SA, biotin–streptavidin; PC, plasma cell.

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