Extended Data Fig. 2: β-glucan treatment promotes granulopoiesis.
From: β-Glucan reprograms neutrophils to promote disease tolerance against influenza A virus
(a) Representative FACS plots for CD64 expression on gated CD11b+Ly6G+ neutrophils at 4 days post β-glucan, and (b) 6 days post-IAV infection. (c) Frequency of Ly6G+ CD64+ macrophages within CD11b+ Ly6G+ cells (n = 5). Mice were treated with β-glucan and immune cells were assessed on day 4 in the lungs. Frequency and total cell count of myeloid cells (d); and adaptive cells (e) in the lungs of β-glucan-treated mice. (f-j) Kinetics of neutrophils in the blood (f) spleen (g, h) and peritoneum (i, j) post β-glucan treatment. FACS plots show indicates the frequency of viable cells. (k) Intravascular staining at day 4 post-β-glucan treatment. Frequency of neutrophils in the vasculature and parenchyma of the lungs (n = 5). Data represented as mean ± SEM. Data were analyzed using one-way ANOVA followed by Sidak’s multiple comparisons tests (f, h, j) or two-way ANOVA followed by Sidak’s multiple comparisons tests (d, e, k). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
