Extended Data Fig. 5: Localization of trained neutrophils is dependent on adaptive immune cells.
From: β-Glucan reprograms neutrophils to promote disease tolerance against influenza A virus
(a-h) C57BL/6 (WT and Rag1-/-) mice were treated with β-glucan. Frequency of neutrophils in the BM (a) and lungs (b); frequency and total cell counts of neutrophils in the peritoneum (c, d); spleen (e, f) at day 4 post-glucan treatment. Frequency of neutrophils in the parenchyma (g) and vasculature (h) of lungs at day 4 post β-glucan treatment (n = 4, data pooled from two individual experiments). (i) Mice were treated with β-glucan, frequency of Rorγt cells CD4+ were quantified at several timepoints post-treatment (n = 5). (j) RORγtGFP/GFP or RORγtWT/GFP mice were treated with β-glucan. Frequency of neutrophils in the BM at day 4 post-glucan treatment (n = 4). (k) RORγtGFP/GFP or RORγtWT/GFP mice were infected with IAV at day 7 post β-glucan. Frequency and total cell counts of neutrophils were quantified in the lung parenchyma (l, m) and vasculature (n, o) at day 6 post-IAV infection (n = 4). Data represented as mean ± SEM. Data were analyzed using one-way ANOVA followed by Sidak’s multiple comparisons tests (i) or two-way ANOVA followed by Sidak’s multiple comparisons tests (a-h, j, l-o). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Illustration in k created using BioRender.com.
