Extended Data Fig. 5: Pcif1 cKO mice exhibit normal phenotypes and retarded tumor growth.

a, A schematic representation of the process for creating conditional knockout Pcif1 mice (Pcif1 cKO) through the crossbreeding of Pcif1^flox/flox mice with Cd4-Cre transgenic mice. b,c, The mRNA and protein level of Pcif1 in CD8 T cells of both WT and Pcif1 cKO mice. n = 3 mice/each group. d-f, The representative images show the body size (d), tissue weight/body weight ratio (e) (n = 6), and body weight (f) (n = 14) of WT or Pcif1 cKO mice. g,h, The assessments of liver function (g) and routine blood tests (h) between Pcif1 cKO mice and their WT littermates. n = 6 mice/each group. i-k, Flow cytometry analysis of thymocytes, peripheral T cells isolated from spleen and lymph nodes of Pcif1 cKO and WT mice. i, j, Percentages of CD4⁺ and CD8⁺ T cells in spleen (i) and lymph nodes (j) of Pcif1 cKO and WT mice. k, Percentages of CD4⁻CD8⁻ double-negative (DN), CD4⁺CD8⁺ double-positive (DP), CD4⁺ single-positive (CD4SP) and CD8⁺ single-positive (CD8SP) cells out of the total number of thymocytes from Pcif1 cKO and WT mice. n = 4 mice/each group. l, Tumor growth of LLC cells was assessed in WT or Pcif1 cKO mice. Tumor volumes of mice were measured every two days. m-p, WT or Pcif1 cKO mice were injected subcutaneously with 10⁵ B16-F10 cells. Tumor growth (m,o), tumor weight (n), and survival (p) were monitored. Each symbol represents an individual mouse. Two-tailed unpaired Student’s t-test (b,e-k,n), two-way ANOVA followed by Sidak’s multiple comparisons test (m), or Kaplan-Meier survival analysis and log-rank (Mantel-Cox) test survival analysis (p) were performed. Data are shown as the mean ± SEM.

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