Extended Data Fig. 4: Gabbr1^ΔCd4 mice display similar colitis symptoms compared with littermate Gabbr1^WT mice.

a, FACS analysis of T_H17 (CD3⁺CD45⁺TCRγδ^-RORγt⁺) and γδT (CD3⁺CD45⁺TCRγδ⁺RORγt^+/-) cells in the small intestine from Gabbr1^WT and Gabbr1^ΔRorc mice small. n = 5 per group. b, Cell number calculation of T_H17 and γδT cells as in a. n = 5 per group. c-e, FACS analysis of ILC1s (c; CD3⁻CD19⁻CD45⁺CD127⁺NKp46⁺NK1.1⁺), ILC2s (d; CD3⁻CD19⁻CD127⁺CD90⁺Sca-1⁺KLRG1⁺) and ILC3s (e; CD3⁻CD19⁻CD45^loCD127⁺RORγt⁺) in the small intestine from Cd4-Cre^-Gabbr1^fl/fl (hereafter Gabbr1^WT) and Cd4-Cre⁺Gabbr1^fl/fl (hereafter Gabbr1^ΔCd4) mice. n = 5 per group. f, Cell number calculation of ILC subsets as in c-e. g-i, Weight change(g), H&E staining assay (h) and histopathological damage evaluation (i) of colon tissues from Gabbr1^WT and Gabbr1^ΔCd4 mice during DSS-induced colitis. Scale bar, 100μm. n = 5 per group. ns, not significant. Data were analyzed by an unpaired two-side Student’s t-test and shown as means ± SD. Data are representative of five independent experiments.

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