Extended Data Fig. 9: Igfbp7^ΔRorc mice show increased proliferation and production in ILC3s.

a, Diagram of colitis induction and Igfbp7 administration strategy. b, c, H&E staining assay (b) and histopathological damage evaluation (c) of colon tissues from indicated mice. Scale bar, 100μm. n = 5 per group. d, FACS analysis of T_H17 (CD3⁺CD45⁺TCRγδ^-RORγt⁺) and γδT (CD3⁺CD45⁺TCRγδ⁺RORγt^+/-) cells in the small intestine from Igfbp7^WT and Igfbp7^ΔRorc mice. n = 5 per group. e, Cell number statistical analysis of T_H17 and γδT cells as in d. n = 5 per group. f-i, FACS analysis of the cell number ILC3 subsets (f, h; CD3⁻CD19⁻CD45^lo gated), proliferation (g, i; left) and IL-17A production (g, i; right) in ILC3s in the small intestine (f, g) and colon (h, i) from Igfbp7^WTRag1^−/−and Igfbp7^ΔRorcRag1^−/−mice. n = 5 per group. j, k, H&E staining assay of small intestine (j) and analysis of small intestinal lymphoid tissues (SILTs) numbers (k) from Igfbp7^WT and Igfbp7^ΔRorc mice. n = 5 per group. ns, not significant. Data were analyzed by an unpaired two-side Student’s t-test and shown as means ± SD. Data are representative of five independent experiments.

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