Extended Data Fig. 2: Gabbr1^ΔRorc mice display increased intestinal ILC3s.

a, Diagram of loxP sequence insertion sites in Gabbr1^flox/flox mice and validation by DNA sequencing. b–d, FACS analysis of ILC3 Gabbr1 expression (b; CD3⁻CD19⁻CD127⁺CD45⁺RORγt⁺gated), ILC1s (c; CD3⁻CD19⁻CD45⁺CD127⁺gated) and ILC2s (d; CD3⁻CD19⁻CD127⁺CD90⁺gated) in small intestines isolated from Rorc-Cre⁻Gabbr1^fl/fl (hereafter Gabbr1^WT) and Rorc-Cre⁺Gabbr1^fl/fl (hereafter Gabbr1^ΔRorc) mice. n = 5 per group. e, Calculation of ILC numbers as in c, d. n = 5 per group. f, g, Immunofluorescent microscopy of ILC3s in the isolated lymphoid follicles (f) and cryptopatches (g) in small intestines from Gabbr1^WT and Gabbr1^ΔRorc mice. Scale bar, 100μm. n = 3 times of experiments were repeated independently with similar results. h, i, H&E staining assay (h) and number statistical analysis (i) of small intestinal lymphoid tissues (SILTs) from Gabbr1^WT and Gabbr1^ΔRorc mice. n = 5 per group. j, k, Flow cytometry assay of the number of ILC3 subsets (CD3⁻CD19⁻CD45^lo gated) in small intestine (j) and colon (k) from Gabbr1^WTRag1^−/−and Gabbr1^ΔRorcRag1^−/−mice. n = 5 per group. l-n, FACS analysis of CLPs (l), CHILPs (m) and ILCPs (n) in bone marrow cells from Gabbr1^WT and Gabbr1^ΔRorc mice. n = 5 per group. o, Cell number statistical analysis of progenitor cells as indicated in l-n. n = 5 per group. ns, not significant. Data were analyzed by an unpaired two-side Student’s t-test and shown as means ± SD. Data are representative of at least independent experiments.

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