Extended Data Fig. 2: T cell presents hyperactive features after NAT10 deletion.

a The fraction of splenic naive (CD44^loCD62L^hi, T_N), effector (CD44^hiCD62L^lo, T_EFF) and memory T cells (CD44^hiCD62L^hi, T_M) were quantified by FCM. Left, representative flow plots. Right, bar graphs showing the percentage of naive, effector, memory T cells in FLOX and CKO mice. n = 3. Exact P values from left to right: *P = 0.0124, *P = 0.0101, *P = 0.0221, *P = 0.0474, NS, P > 0.05. b T cell activation measured by CD69 and CD44 staining of activated FLOX and CKO T_N with anti-CD3/28 stimulation for 24 h. Left, representative flow plots. Right, bar plots showing the percentage of CD44⁺, CD69⁺ and CD44⁺CD69⁺ T cells in FLOX and CKO CD8⁺ and CD4⁺ T cells, respectively. FLOX n = 4, CKO n = 3. Exact P values from left to right: ****P < 0.0001, ***P = 0.0002, *P = 0.0259, **P = 0.0028, *P = 0.0128. c Expression of IFNγ in FLOX and CKO T cells measured by FCM. Left, representative flow plots. Right, bar graphs showing IFNg⁺ fraction as well as IFNγ MFI in FLOX and CKO splenic T cells. n = 4. ***P (CD8⁺T IFNγ MFI) = 0.0009, ***P (CD8⁺ IFNγ⁺T%) = 0.0004, **P = 0.0056, *P = 0.024. d Expression of GZMA in CD8⁺ FLOX and CKO T cells measured by FCM. Left, representative flow plots. Right, bar graphs showing GZMA⁺ fraction and GZMA MFI of CD8⁺ FLOX and CKO T cells. n = 3. *P = 0.0100. e Expression of GZMB in CD8⁺ FLOX and CKO T cells measured by FCM. Left, representative flow plots. Right, bar graphs showing GZMB⁺ fraction and GZMB MFI of CD8⁺ FLOX and CKO T cells. n = 3. **P = 0.0050; *P = 0.0138. f Expression of TNF in CD8⁺ FLOX and CKO T cells measured by FCM. Left, representative flow plots. Right, bar graphs showing TNF⁺ fraction and TNF MFI of CD8⁺ FLOX and CKO T cells. n = 4. *P = 0.0433. g Expression of perforin in CD8⁺ FLOX and CKO T cells measured by FCM. Left, representative flow plots. Right, bar graphs showing the percentage of perforin⁺ CD8⁺ FLOX and CKO T cells. n = 4. ***P = 0.0002. The values of “n” all refer to biologically independent samples. Error bars represent mean ± s.e.m. (a–g). Two-tailed, unpaired t-test (a–g).

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