Extended Data Fig. 3: NAT10 is required for the stability and translation efficiency of Myc mRNA.
a Gene Ontology (GO) enrichment analysis of down-regulated genes in CKO T cells compared to their FLOX controls. Biological process terms were displayed. b KEGG pathway analysis of mRNAs with lower ac4C levels in CKO T cells compared to their FLOX controls. c GSEA plots showing significantly enriched gene sets, “DNA replication”, “G1 S transition” and “Positive regulation of cell cycle”. d Cumulative distribution plots of translation efficiency (TE) for ac4C (−)/(+) transcripts in FLOX CD3+ T cells (upper left), and ac4C (−) transcripts in FLOX and CKO T cells (upper right). The cumulative distribution plot of TE relative changes (log fold change, CKO VS FLOX) for ac4C (−) and ac4C (+) transcripts was also displayed (down). Hypergeometric test, one-sided, BH adjusted (a, b), two-tailed Kolmogorov–Smirnov test (c) and two-tailed Mann-Whitney U test (d).
