Extended Data Fig. 6: Exploration of reduced NAT10 levels in immune senescence.
a Schematic diagram of RNA-seq and ac4C-RIP-seq performed on spleen from young (8w) and aged (72w) mice. b GO enrichment analysis of transcripts with less ac4C modification in splenocytes from old mice compared to their young controls. Biological process terms were displayed. c Bar graph showing ac4C peak distribution on mRNAs of splenocytes from young (8w) and old (72w) mouse. d Motif analysis for ac4C peaks in spleen from young (8w) and old (72w) mice. e GO enrichment analysis of the overlapped genes between “Old VS Young mRNA down” (n = 679) and “Old VS Young ac4C down” (n = 1586) genes. Biological process terms were displayed. f Schematic diagram showing isolation and culture procedure of PBMC. g Percentages of CD3+ T cells in PBMC from young (<60 years old) and old (≥60 years old) subjects with anti-CD3/28 stimulation for 48 h. Left, representative flow plots. Right, bar graph displaying the percentage of CD3+T cells after 48h-culture (n = 5 biologically independent samples.). Error bars represent mean ± s.e.m., NS P > 0.05 by two-tailed, unpaired t-test. h UMAP visualization of PBMC from 33 COVID-19 patients and 8 healthy controls according to scRNA-seq data from Wilk, Aaron J et al.22. i Deeper UMAP visualization of NK and T cells identified in (h). Hypergeometric test, one-sided, BH adjusted (b, d, e). Icons in Extended Data Fig. 6a, f were from Figdraw (https://www.figdraw.com/) with the agreement ID: RIARO787e2.
