Fig. 6: NAT10 matters in T cell antiviral immunity.
a, Schematic of the acute LCMV infection model in FLOX and CKO mice. Eight-week-old FLOX and CKO mice were intraperitoneally injected with 2 × 105 p.f.u. LCMV Armstrong and killed at 8, 16 and 24 days after infection. Animals treated with equal volumes of PBS served as blank controls. Serum, spleens and lymph nodes from the LCMV group were collected for further analysis. b, Viral loads in the serum derived from FLOX (n = 9) and CKO (n = 10) mice at 8 days after LCMV administration; *P = 0.0253. c, Gross appearance of spleens and inguinal lymph nodes from infected FLOX and CKO mice and their blank controls at 8 days after PBS or virus administration. d, Absolute number of splenic CD8+ and CD4+ T cells in FLOX (n = 8) and CKO (n = 10) infected mice at 8 days after infection; ****P < 0.0001. e,f, Percentages of GP33+CD44+ CD8+ T cells in the spleen, lymph node and blood from FLOX (n = 6, 6 and 6) and CKO (n = 4, 4 and 3) infected mice at 8 days after infection; ****P < 0.0001 and ***P = 0001. g, Absolute number of splenic GP33+CD44+ CD8+ T cells from FLOX (n = 6) and CKO (n = 4) infected mice at 8 days after infection; ****P < 0.0001. h, Absolute number of splenic IFNγ+ and IFNγ+TNF+ CD8+ T cells in FLOX (n = 8) and CKO (n = 9) infected mice at 8 days after infection; ****P < 0.0001 and ***P = 0.0006. i, Serum IL-2, IFNγ and TNF (8, 4 and 4 days after infection, respectively) levels in FLOX and CKO infected mice (n = 6); ****P < 0.0001, ***P = 0.0007 and *P = 0.0141. j, Percentages of GP33+CD44+ CD8+ T cells in blood from FLOX (n = 5, 6 and 6) and CKO (n = 3, 5 and 5) infected mice at 8, 16 and 24 days after infection; ****P < 0.0001. k, Percentages of GP33+ CD8+ T cells (FLOX n = 6, CKO n = 5) and KLRG1loCD127hiGP33+ CD8+ T cells (FLOX n = 6, CKO n = 3) from FLOX and CKO infected mice at 24 days after infection; ****P < 0.0001 and ***P = 0.0003. n refers to the number of biologically independent samples. Error bars represent mean ± s.e.m. (b, d and f–k). Data were analyzed by two-tailed, unpaired t-test (b, d, f–i and k) or two-tailed, two-way ANOVA with Šídák’s multiple comparisons test (j).
