Extended Data Fig. 4: Single-cell characterization of the acute hyperinflammatory response to LPS administration in peripheral blood and bone marrow cells.

a, Violin plot of marker genes of all mononuclear immune cell types identified in peripheral blood and bone marrow obtained from LPS-challenged healthy volunteers (n = 3 for each compartment) at baseline (BL, before the LPS challenge) and at 4 h and d7 post-LPS challenge. b, Sample composition (in percentage) for each cell type depicted for each donor and timepoint as in a. c, Overlayed and separated UMAP of all cells in blood and bone marrow obtained from LPS-challenged healthy volunteers (n = 3 for each compartment) at BL, and at 4 h and d7 post-LPS challenge, colored based on acquisition time point (upper) and compartment (bottom). d, Violin plot of marker genes HSCs and myeloid cell types identified in blood and bone marrow obtained from LPS-challenged healthy volunteers (n = 3 for each compartment) at BL and at 4 h and d7 post-LPS challenge. e, Overlayed and separated UMAP of HSCs and myeloid cells in blood and bone marrow obtained from LPS-challenged healthy volunteers (n = 3 for each compartment) at BL, and at 4 h post-LPS challenge, colored based on acquisition time point (upper) and compartment (bottom). f, Percentage of each monocytic cell type in blood and bone marrow obtained from LPS-challenged healthy volunteers (n = 3 for each compartment) at BL and 4 h post-LPS, separated based on compartment.