Extended Data Fig. 5: Single-cell characterization of the acute hyperinflammatory response to LPS administration in peripheral blood and bone marrow HSC & myeloid cells.
From: Systemic inflammation impairs myelopoiesis and interferon type I responses in humans
a, Scatterplot of normalized overall gene expression profiles of peripheral blood (x-axis) and bone marrow (y-axis) monocytes obtained from healthy volunteers (n = 3) at baseline (before LPS administration). b, Scatterplot of normalized baseline monocytic genes (genes highly expressed in monocytes) in blood (x-axis) and bone marrow (y-axis) monocytes obtained from healthy volunteers (n = 3) at baseline (before LPS administration). c, Percentage of each monocytic cell type in blood and bone marrow obtained from healthy volunteers (n = 3) at baseline (before LPS administration), colored based on compartment. d, Normalized expression of differentially expressed genes in blood and bone marrow monocytes obtained from healthy volunteers (n = 3) at 4 h post-LPS vs. baseline (BL). e, Scatterplot of logn(fold change) in differentially expressed genes in blood (x-axis) and bone marrow (y-axis) monocytes obtained from healthy volunteers (n = 3) at 4 h post-LPS vs. baseline. f, UMAP representation of identified 5 gene sets in blood and bone marrow HSC & myeloid cells of LPS-challenged healthy volunteers (n = 3 for each compartment) at baseline and at 4 h post-LPS challenge, with relative enrichment on single cell level. g, Heatmap of average enrichment of 5 identified gene sets in blood and bone marrow HSC & myeloid cells of LPS-challenged healthy volunteers (n = 3 for each compartment) at baseline and at 4 h post-LPS challenge per cell type and timepoint. h. Heatmap representation of infMono genes in myeloid cells of LPS-challenged healthy volunteers (n = 3 for each compartment) at baseline and at 4 h post-LPS challenge. i, UMAP representation of neutrophils from a publicly available healthy subjects peripheral blood neutrophil dataset. j, Violin plot of gene expression profiles of neutrophil-enriched genes in blood and bone marrow HSC & myeloid cells of LPS-challenged healthy volunteers (n = 3 for each compartment) at baseline and at 4 h post-LPS challenge.
