Extended Data Fig. 7: LPS-induced gene programs in patients with early sepsis and impaired monocyte maturation in the late immunosuppressive phase following LPS administration.

a, UMAP representation of enrichment of sepsis specific MS1 (MDSC) gene signature defined in LYZ⁺ monocytes of patients with early sepsis (left panel) and in HSCs and myeloid cells in blood and bone marrow obtained from LPS-challenged healthy volunteers (n = 3 for each compartment) at baseline (before LPS administration) and at 4 h post-LPS challenge (right panel). b, Overlayed and separated UMAP of HSCs and myeloid cells in peripheral blood and bone marrow obtained from LPS-challenged healthy volunteers (n = 3 for each compartment) at BL, and at 4 h and d7 post-LPS challenge, colored based on compartment (left panels) and acquisition time point (right panels). c, Cell type proportion (percentage) of HSC and myeloid lineage cells in peripheral blood and bone marrow obtained from LPS-challenged healthy volunteers (n = 3 for each compartment) at BL and at d7 post-LPS challenge. d, Bar plot of log2(relative abundance [percentage] of each cell type) change in HSC and myeloid lineage cells in peripheral blood and bone marrow obtained from LPS-challenged healthy volunteers (n = 3 for each compartment) at BL and at d7 post-LPS challenge. e, UMAP of intermediate and non-classical monocytes in HSC and myeloid lineage cells in peripheral blood and bone marrow obtained from LPS-challenged healthy volunteers (n = 3 for each compartment) at baseline and at d7 post-LPS challenge. f, UMAP representation of normalized single-cell gene expression of several IFN-I signaling pathway and NCM-enriched genes in HSC and myeloid lineage cells in peripheral blood and bone marrow obtained from LPS-challenged healthy volunteers (n = 3 for each compartment) at baseline and at d7 post-LPS challenge.