Extended Data Fig. 2: IFN signaling impairment in blood monocytes in the late immunosuppressive phase following LPS administration.

a, Scatterplot of gene expression in blood CD14⁺ monocytes at 4 h, 8 h, 24 h, d7, and d7 + 4 h following LPS administration (y-axis) versus baseline (before LPS administration, x-axis) in healthy volunteers. Dots represent the average expression of n = 3 replicates. Red dots indicate significantly up-regulated genes (fold-change > 2 & q value < 0.05), while blue dots depict significantly down-regulated genes (fold-change < 0.5 & q value < 0.05). b, log2(normalized expression) of MHC class II (HLA-II) genes in blood CD14⁺ monocytes at baseline (BL, before LPS administration) and at 4 h, 8 h, 24 h, d7, and d7 + 4 h following LPS administration in healthy volunteers (n = 3). c, Gene set enrichment analysis of interferon signaling genes in blood CD14⁺ monocytes of LPS-challenged healthy volunteers (n = 3, based on the d7 vs. BL comparison). d, log2(relative normalized expression) of interferon signaling genes in blood CD14⁺ monocytes of LPS-challenged healthy volunteers (n = 3, ordered and colored based on relative expression of d7 vs. BL).