Extended Data Fig. 3: IL-10 decreases chromatin accessibility and preferentially suppresses IRF activity.
a–e, Additional analysis of ATACseq data shown in Fig. 3 obtained using monocytes from 4 independent donors and treated as depicted in Extended Data Fig. 1a. a, Hallmark pathway enrichment analyses of genes associated with peaks identified as suppressed or non-suppressed in Fig. 3b. b, Heatmap of the H3K27ac normalized signal density surrounding LPS-induced ATAC peaks identified as suppressed or non-suppressed and plotted under the indicated conditions. Results are presented in RPKM values within a range of ± 3.0 kb around peak centers. c–e, Volcano plot of differential binding analysis of JASPAR motifs by TOBIAS using BINDetect algorithm c, for peaks associated with IL-10, LPS, and IL-10 + LPS compared to resting control, d, for TNF vs. IL-10 + TNF, e, for TNF, and IL-10 + TNF treated human monocytes compared to resting control. Created in https://BioRender.com.
