Extended Data Fig. 4: IL-10 broadly suppresses LPS-induced IRF1 binding and associated histone acetylation.
a–d, Additional analysis of IRF1 CUT&RUN data shown in Fig. 4 obtained using monocytes from 3 independent donors and treated as depicted in Extended Data Fig. 1a. a, Bar plot depicting total number of IRF1 binding sites from CUT&RUN experiments. b, PCA plot of IRF1 CUT&RUN experiments for Resting, IL-10, LPS, and IL-10 + LPS treated primary human monocytes. c, Hallmark pathway enrichment analyses of genes associated with LPS-induced IRF1 binding peaks. d, Representative IGV gene tracks are shown, illustrating LPS-induced IRF1 binding, H3K4me3 and H3K27ac peaks, and ATACseq peaks at the OAS3 locus. Created in https://BioRender.com.
