Extended Data Fig. 5: EOS301984 restores the expansion of functional antigen-specific CD8+ T cells in the presence of high concentrations of adenosine.
From: Inhibition of ENT1 relieves intracellular adenosine-mediated T cell suppression in cancer
a, Expansion of NLVPMVATV-specific CD8+ T cells in the presence of the peptide, IL-2 and IL-7. Representative of 5 donors. b, ENT1 and Ki-67 expression in tet+ and tet- CD8+ T cells. Representative of 2 donors. c-d, FluoroSpot analysis of IFNγ and granzyme B production, respectively, of PBMC cultures generated as in Fig. 4i. Representative of 3 donors. e, Summary analysis of granzyme B FluoroSpot data from cultures generated as in Fig. 4i. Symbols represent each donor (biological replicates), bars are group mean ± s.d. Two-way ANOVA with Tukey’s multiple comparisons test.
