Extended Data Fig. 6: Antigen-specific CD8+ T cells expanded in the presence of high concentrations of adenosine by EOS301984 retain the capacity to kill antigen-bearing target cells.
From: Inhibition of ENT1 relieves intracellular adenosine-mediated T cell suppression in cancer
a, T2 cells were stained either with a low concentration of eFluor450 and then pulsed with NLVPMVATV peptide, or stained with a high concentration and left non-pulsed. Gating based on FSC-A vs SSC-A and eFluor450 distinguishes T2 cells from unstained PBMCs. b, T2 cells pulsed with NLVPMVATV peptide are selectively killed by PBMC populations enriched for NLVPMVATV-specific CD8+ T cells whilst non-pulsed T2 cells are spared. Representative of 3 donors. c, PBMC populations generated as in Fig. 4i were used in NLVPMVATV peptide-specific T2 killing assays. Representative of 3 donors. d, Summary data from (c). Bars represent the mean value ± s.d. from technical triplicates. Two-way ANOVA with Tukey’s multiple comparisons test. Representative of 3 donors. e-g, Pearson correlation of normalized specific killing activity and frequency of pp65-specific CD8+ T cells, IFNγ SFU, or granzyme B SFU, respectively. 95% confidence bands of the best-fit line from simple linear regression indicated. Data pooled from 3 donors, p-values are two-tailed.
