Fig. 2: ENT1 deficiency leads to strong anti-tumor immunity in various mouse cancer models.
From: Inhibition of ENT1 relieves intracellular adenosine-mediated T cell suppression in cancer
a–c, Tumor growth curves and survival (defined as tumor volume less than the lower of WT mean at study end or 2,000 mm3) analysis of MCA205 (a), MC38 (b) and Pan02 (c) subcutaneous tumors in WT and ENT1-KO mice from one experiment for each model. Number of mice with complete tumor regression (CR) noted where relevant. TGI, tumor growth inhibition. d, Adenosine concentrations in tumor interstitial fluid derived from MCA205, MC38 and Pan02 subcutaneous tumors implanted in WT mice (n = 10 in all cases), as indicated. Symbols represent individual mice; bars represent group mean value; error bars, s.d. e, MC38 tumor growth curves and survival (defined as tumor volume less than WT mean at study end) analysis in WT, ENT1-KO mice and ENT1-KO mice following CD8+ T cell depletion. f, Tumor growth curves and survival (defined as tumor volume of <2,000 mm3) analysis of rechallenge experiments following MC38 tumor growth in WT and ENT1-KO mice with primary inoculation and secondary inoculation in n = 7 complete regressors from the ENT1-KO group. Representative of two experiments. g, Tumor growth curves and survival (defined as tumor volume less than WT mean at study end) analysis of KPC subcutaneous tumors in WT and ENT1-KO mice from one experiment. h–j, Profiling of CD8+ T cell infiltration (h), Ki-67 expression (i) and IFNγ production (j) following ex vivo restimulation of CD8+ TILs of WT and ENT1-KO mice bearing KPC tumors (see also Supplementary Fig. 2). Mean values displayed; error bars, s.d. P values from two-tailed unpaired t-test, n = 8 per group from one experiment. In a–c, e and g, mean values are displayed; error bars, s.e.m.; P values are from two-way ANOVA of log-transformed tumor volumes with Šídák’s multiple comparisons test on days 25, 26, 29, 21 and 27, respectively. Kaplan–Meier survival analysis with log-rank (Mantel–Cox) test (a–c,e–g) corrected for multiple comparisons with the Bonferroni method (e). All mice were C57BL/6 females, 8 weeks of age at tumor inoculation.
