Extended Data Fig. 2: DNase I injections blunt alum-induced autoimmunity.
a, Treatment and analysis scheme of male B6 mice in three independent biological experiments. b, Spleen gross morphology, mass to body weight ratio (n = 9/PBS, n = 10/alum, n = 12/alum+DNase I) and absolute splenocyte numbers (n = 5/PBS, n = 7 alum, n = 5/alum+DNase I). Scale bar, 1 cm. c, CLIFT assay of serum anti-dsDNA IgG. Scale bar, 20 μm. d, FC plots of anti-mtDNA antibody forming cells (AFC) in spleens. e, GC B cell number (n = 5/PBS, n = 7/alum, n = 5/alum+DNase I) and frequencies of PC (n = 7/PBS, n = 9/alum, n = 7/alum+DNase I) and MZ B cells (n = 9/PBS, n = 9/alum, n = 12/alum+DNase I) in the spleens. f, Percentages of splenic proliferating Ki67+ B220+cells and CD4+ T cells. g, ELISA of serum IL-21, IL-6, IL-1β and IFN-α. h, Relative amounts of circulating mtDNA. i, 8-Oxo-dG containing DNA amounts (n = 5/PBS, n = 7/alum, n = 5/alum+DNase I) and ratio of Fpg-treated (+) to nontreated (−) circulating mtDNA, indicating the fraction of non-Ox-mtDNA (Fpg-resistant) in mtDNA (D-loop, Cox1 and Non-Numt, n = 9/group). j, Ratio of serum nDNA (Tert or B2m) to mtDNA (D-loop). Results in (b and e-j) are mean ± SD. n = 9/PBS, n = 9/alum, n = 12/alum+DNase I (f and g). n = 7/PBS, n = 9/alum, n = 7/alum+DNase I (h and j). Kruskal–Wallis test. Micrographs (b and c) are representative of at least three independent experiments.
