Extended Data Fig. 5: Alum induced ccf-Ox-mtDNA is sensed by functionally important peritoneal pDC.
a-f, Relative amounts of peritoneal mtDNA (a,c and e), and non-Ox-mtDNA (Fpg-resistant) (b,d and f) 24 h after PBS or alum injection into the indicated mice in Fig. 3f, g and i. (n = 7/WT PBS, n = 5/WT alum, n = 7/Nlrp3−/− alum (a); n = 7/WT PBS, n = 4/WT alum, n = 9/Gsdmd−/− alum (c); n = 8/WT PBS, n = 8/Cmpk2ff alum, n = 9/Cmpk2ΔMye alum). g, Treatment and analysis scheme (short-term). BDCA2-DTR− and BDCA2-DTR+ male mice were i.p. injected with DT (100 ng/mice), followed by PBS (n = 6/BDCA2-DTR− and n = 4/BDCA2-DTR+) or alum (n = 6/genotype) injections and FC analyses of splenic and peritoneal pDC after 24 h. h, Treatment and analysis scheme (long term) for i-n. Alum-treated BDCA2-DTR− and BDCA2-DTR+ female mice were repetitively injected with PBS (n = 5/BDCA2-DTR- and n = 4/BDCA2-DTR+) or alum (n = 8/genotype) and analyzed. i, Spleen mass to body weight ratio. j, k, ELISA of serum anti-dsDNA and anti-nucleosome IgGs (j), IL-21 and IFN-α (k). l, IF of GL7 (blue) and B220 (red) stained spleen sections. Scale bar, 50 μm. m, FC frequencies of splenic GC B, Tfh, IgG1+IgM-CD19+ and IgG2b+IgM-CD19+ cells. n, Sizes of glomeruli (n = 130/PBS/genotype, n = 125/BDCA2-DTR− alum, n = 122/BDCA2-DTR+ alum) in H&E-stained kidney sections, MFI of kidney IgG deposits (n = 112/PBS/genotype, n = 118/BDCA2-DTR− alum, n = 103/BDCA2-DTR+ alum) and urinary protein ELISA (n = 5/BDCA2-DTR− PBS, n = 4/BDCA2-DTR+ PBS, n = 8/BDCA2-DTR− or BDCA2-DTR+ Alum). Results in (a-g, i-k, m and n) are mean ± SD. n = 5/BDCA2-DTR− PBS, n = 4/BDCA2-DTR+ PBS, n = 8/BDCA2-DTR− or BDCA2-DTR+ Alum (i-m). Two-way ANOVA with Tukey multiple-comparison test. Micrographs (l) are representative of at least three independent experiments.
