Extended Data Fig. 8: MKRN2 form complex with PABPC1, PAIP1 in an Il6 mRNA dependent manner.
From: The RNA-binding E3 ligase MKRN2 selectively disrupts Il6 translation to restrain inflammation
Immunoblot showing the level of GST and His fusion protein in GST pull-down complex. 1 μg GST or GST-MKRN2 was co-incubated with 1 μg recombinant His-PAIP1, His-PABPC1 and Il6 transcript for 4 h as indicated. Sepharose 4B beads were then used to pull down the GST complex.
