Extended Data Fig. 2: MKRN2 does not affect the mRNA levels of innate immune cytokines.
From: The RNA-binding E3 ligase MKRN2 selectively disrupts Il6 translation to restrain inflammation
a, Quantification of the mRNA levels of Il6, Tnf and Ifnb1 by Q-PCR in Mkrn2+/+ and Mkrn2−/− BMDMs treated with LPS (100 ng/ml) for 0, 2, 4, 8, 12, or 24 h as indicated (n = 3). b, ELISA showing the concentrations of IL-6, TNF and IFN-β in the supernatants of Mkrn2+/+ and Mkrn2−/− DCs treated with LPS (100 ng/ml) for 0, 4, 8, or 12 h (n = 3). c, Quantification of the mRNA levels of Il6, Tnf and Ifnb1 by Q-PCR in Mkrn2+/+ and Mkrn2−/− DCs treated with LPS (100 ng/ml) for 0, 2, 4, 8, 12, or 24 h as indicated (n = 3). Results are presented as mean ± SD of three biological replicates (one-way ANOVA followed by Bonferroni multiple comparison). n.s. not significant; ****P < 0.0001.
