Extended Data Fig. 2: Flow cytometry and GO enrichment analyses of Cx3cr1GFP/+ mouse liver cells.
From: Neuroprotective liver portal area macrophages attenuate hepatic inflammation
(a) Flow cytometric analysis of liver LPAMs and LCMs gated on CD45+CX3CR1+ cells in whole Cx3cr1GFP/+ mice liver NPCs. (b) Flow cytometric analysis of liver LPAMs and LCMs gated on CD45+CX3CR1+ cells in vascular-enriched Cx3cr1GFP/+ mice liver NPCs. (c) The fluorescence minus one (FMO) control (without CD207 or CD63) for the flow cytometric gated strategy of LPAMs and LCMs. (d) Gene Ontology (GO) enrichment analysis in Pmepa1+Zmynd15+ LPAMs, Gpnmb+Spp1+ LPAMs, LCMs, Il1rn Macs and Ptprb Macs. (e) CD63 expression in human liver section: https://www.proteinatlas.org/ENSG00000135404-CD63/tissue/Liver#img. CD68 expression in human liver section: https://www.proteinatlas.org/ENSG00000129226-CD68/tissue/liver#img. C1QC expression in human liver section: https://www.proteinatlas.org/ENSG00000159189-C1QC/tissue/liver#img. HLA-DR expression in human liver section: https://www.proteinatlas.org/ENSG00000204287-HLA-DRA/tissue/liver#img. VSIG4 expression in human liver section:https://www.proteinatlas.org/ENSG00000155659-VSIG4/tissue/liver#. The green arrows indicated KCs in the hepatic sinusoid and the red arrows indicated portal macrophages.
