Extended Data Fig. 4: The response of LPAMs upon antigen or bacteria challenge.
From: Neuroprotective liver portal area macrophages attenuate hepatic inflammation
(a) Imaging of LPAMs capturing hepatocyte-derived antigens. Scale bars, 20 µm. CX3CR1+ LPAMs in green, tfRFP-expressing HepG2 cells in magenta. The blue arrow indicates LPAMs that have phagocytosed tfRFP. (b) Apoptotic Raji cells (Raji-mcherry) were injected into Cx3cr1GFP/+ mice. Flow cytometry was used to detect the phagocytosis of cellular debris by LPAMs and KCs. The statistical results of the mean fluorescence intensity (MFI) of antigen and dead cells captured by LPAMs and KCs, as detected by flow cytometry, n = 4 per group. Mean ± SD, two-tailed unpaired t-test. (c) Imaging of LPAMs and KCs in the liver during infection with tfRFP-expressed E.coli-tfRFP. CX3CR1+ cells in green, F4/80 in white and E.coli-tfRFP in magenta. (d) GSEA analysis of the ‘Defense response to bacterium’ pathway between LPAMs versus LCMs, and LPAMs versus KCs. The top 20 genes with expression differences are listed on the right panel. The imaging data in a, c were from representative experiments.
