Extended Data Fig. 9: Verification of LPAM depletion in Cx3cr1CreER-iDTR mice.
From: Neuroprotective liver portal area macrophages attenuate hepatic inflammation
Cx3cr1CreER-iDTR mice were injected with different doses of TAMO (3 mg/kg, 15 mg/kg, and 75 mg/kg) and 50 µg/kg DT. Cx3cr1CreER-iDTR mice injected with TAMO and PBS were used as controls. (a-b) Gating strategy for LPAM (CX3CR1+MHC-II+CD63+), LCM (CX3CR1+MHC-II+CD207+), monocyte (CX3CR1+MHC-II-F4/80+), and DC (MHC-II+CD11c+CD64−) in the liver. (c) The depletion efficiency of LPAM, LCM, and monocyte in LPAMCtr and LPAMDel mice, n = 8 (Control, 15 mg/kg), n = 4 (3 mg/kg, 75 mg/kg). Mean ± SEM, two-tailed unpaired t-test. (d) The depletion efficiency of liver DCs in LPAMCtr and LPAMDel mice, n = 4 (Control), n = 3 (3 mg/kg, 15 mg/kg, 75 mg/kg). Mean ± SEM, two-tailed unpaired t-test. (e) The depletion efficiency of other tissue macrophages in LPAMCtr and LPAMDel mice, n = 4 in each group of lung and spleen Mac, n = 3 in each group of intestine Mac. Mean ± SEM, two-tailed unpaired t-test. (f-g) The biochemical analysis of liver ALT and AST, n = 7 (TAMO + PBS), n = 6 (TAMO + DT), n = 11 (MCD LPAMCtr), n = 8 (MCD LPAMDel). The data were pooled from at least two experiments (mean ± SEM, two-tailed unpaired t-test). (h-i) H&E and NAS score for NASH liver tissue, n = 5 (MCD LPAMCtr), n = 6 (MCD LPAMDel), n = 5 (MCD LPAMcCtr), n = 4 (MCD LPAMcDel). Mean ± SD, two-tailed unpaired t-test. The imaging data in g were from representative experiments.
