Extended Data Fig. 6: Composition of the TME in mice injected with either OLFR644-/- or VMN2R29-/- macrophages.
From: Chemosensor receptors are lipid-detecting regulators of macrophage function in cancer
a-f) FACS analysis to determine the immune infiltrate of tumors upon injection with LGP (n = 8), OLFR644-/- (n = 8) and VMN2R29-/- (n = 8) macrophages: a) Quantification of macrophages gated on CD45+ cells. b-c) % cells gated on GFP-F4/80 + CD11b+ cells. (d-f) % cells gated on CD45+ cells. g-i) The response of splenocytes to mitomycin c killed tumor cells was examined ex vivo using tumor cell restimulation assays. Interferon production in response to stimulation was assessed through ELISA (LGP n = 7, OLFR644 n = 5 and VMN2R29 n = 6) (h) or FACS analysis (LGP n = 6, OLFR644 n = 7 and VMN2R29 n = 8. (i) after a 72-hour incubation period. j)FACS analysis of interferon production in splenocytes from mice contextually injected orthotopically with Pten-/-; Trp53-/- and with LGP-Macs, OLFR644-/-Macs or VMN2R29-/- Macs. k-l) FACS analysis of CD8 + T lymphocytes to confirm the CD8 depletion in mice treated with antiCD8 antibody or isotype in (k) blood or (l) tumor. Events are gated on CD3+ cells. Statistical analyses were performed using two-tailed unpaired Student’s t test. Values are presented as mean ± SEM.
