Extended Data Fig. 7: Composition of the TME in mice injected with macrophages exposed to 13-cRA.
From: Chemosensor receptors are lipid-detecting regulators of macrophage function in cancer
a-d) FACS analysis to determine the immune infiltrate of tumors upon injection with macrophages pre-treated for 4 hours with DMSO or 13-cRA: (a) Quantification of macrophages gated on CD45+ cells. (b) % of monocytes gated on CD45+ cells. (c-d) % of dendritic cells gated on CD45+ cells (a-d Untr, Macs+DMSO or 13cRA n = 10). e-j) FACS analysis to determine the immune infiltrate of tumors upon injection with OLFR644-/- and VMN2R29-/- macrophages pre-treated for 4 hours with DMSO or 13-cRA: e) Quantification of macrophages gated on CD45+ cells. f-g) % cells gated on GFP-F4/80 + CD11b+ cells. (h-j) % cells gated on CD45+ cells. (E-J: LGP n = 6, LGP+13cRA n = 6, OLFR644 n = 7, OLFR644 + 13cRA n = 6). Statistical analyses were performed using two-tailed unpaired Student’s t test. Values are presented as mean ± SEM.
