Extended Data Fig. 8: Expression of OR51E2 by human macrophages.

a) OR51E1 and OR51E2 expression in PCa human tissues respect to normal tissue. TGSA data were analysed using GEPIA2 (n tum= 492, n Normal= 152) and presented as Min to Max box-and-whisker plot, the box extends from the 25th to 75th percentiles and the whiskers reach the sample maximum and minimum values, the median is indicated at center line. b) Representative confocal immunofluorescence images and quantification of human PCa tissues (patient#2) showing the expression of OR51E2 (red) in CD68+ macrophages (green). Images were acquired with an SP8-II confocal microscope (Leica). Scale bar: 10um. c) Representative confocal immunofluorescence images showing PBMC- derived macrophages (CD68 + , green) expressing OR51E2 (red). Nuclei were counterstained with DAPI (blue). Images were acquired with an SP8- II confocal microscope (Leica) with a 40× objective. Images on the right are 2× digital zoom. d) Flow cytometry analysis to assess the impact of gene silencing on murine bone marrow derived macrophages. Control macrophages (LGP) were compared to OLFR78-KO (Or51e2-KO) macrophages after exposure to Pten-/-; Trp53-/- conditioned media. Cells were gated on F4/80 + CD11b+ cells. e) Schematic representation of the plasmid encoding OR, RTP1S, CRE-luciferase and 5 pRL-SV40. f-g) Ca2+ flux in THP1 cells in absence or presence of partial genetic deletion of OR51E2 KO treated with (f) β-ionone (100 µM) (Ctrl n = 6, treated n = 8), (g) palmitic acid (100 µM) (Ctrl n = 10, treated n = 11), or (h) ionomycin (2 µM) (Ctrl n = 3, treated n = 3). i) Ca2+ flux in primary macrophages in absence or presence of partial genetic deletion of OR51E2 KO treated with ionomycin (2 µM) (Ctrl n = 3, treated n = 3). Values are presented as mean ± SEM.