Extended Data Fig. 9: Expression of OR51E2 by human macrophages.

a) Gene set enrichment analysis (GSEA) showing up and downregulated biological pathways in BPMCs derived macrophages exposed or not to Palmitic acid. The size of each dot indicates the number of enriched genes relative to the pathway of interest. The fraction of genes represents the proportion of genes significantly enriched out of the total number of genes of the pathway. b) Volcano plot showing differential expressed genes in palmitic acid-exposed macrophages compared to untreated macrophages. Genes are colored according to their log2 fold change value (Blue <=−0.5, red >=+0.5). c-f) FACS analysis of primary macrophages exposed to the conditioned media from PC3 tumor cells or to palmitic acid (100 nM) in presence or absence of partial genetic deletion of OR51E2. Bar graphs showing the % of (c) HLA-DR+ (Ctrl n = 4, CM n = 6, PA n = 6, siRNA ctrl n = 4, siRNA CM n = 5, siRNA PA n = 4), (d) CD204+ (Ctrl n = 4, CM n = 4, PA n = 6, siRNA ctrl n = 4, siRNA CM n = 5, siRNA PA n = 4), (e) CD206+ (Ctrl n = 4, CM n = 6, PA n = 6, siRNA ctrl n = 4, siRNA CM n = 4, siRNA PA n = 4) and (f) Arginase1+ (Ctrl n = 5, CM n = 5, PA n = 4, siRNA ctrl n = 4, siRNA CM n = 4, siRNA PA n = 4) gated on CD68+ macrophages. g) Absolute count of different cell subsets in non-tumor (n = 7) and tumor (n = 9) regions. h) H&E and spatial analysis of the distribution of palmitic acid by mass spectrometry imaging in patient number 3. i) Spatial distribution of Inflamm-TAMs, IFN TAMs, LA-TAMs, Angio-TAMs and Reg-TAMs signature in patient number #3. Statistical analyses were performed using two-tailed unpaired Student’s t test. Values are presented as mean ± SEM.