Fig. 5: Pharmacological inhibition of olfactory receptor in a prostate cancer model.

a, FACS analysis of LGP (n = 3), OLFR644-KO (n = 3) and VMN2R29-KO (n = 3) macrophages exposed to 13-cRA for 4 h with and without Pten^−/−Trp53^−/− conditioned media. Events are plotted as ratio versus conditioned macrophages. b,c, Experimental scheme (b) and tumor growth (c): mice were injected intravenously with macrophages pretreated for 4 h with DMSO (n = 8) or 13-cRA (n = 9). Ctrl mice were untreated. c, Tumor growth expressed as a percentage of the initial volume. d, Representative FACS plot of GFP⁻ and GFP⁺ tumor-infiltrating macrophages. e,f, FACS analysis: percentage of cells gated on GFP⁺F4/80⁺CD11b⁺ cells (Macs + DMSO or 13-cRA, n = 8) (e); percentages of CD39⁺ cells gated on CD8⁺ T cells and FoxP3⁺CD25⁺ T_reg cells gated on CD4⁺ T cells (Untr., Macs + DMSO or 13-cRA, n = 9) (f). g, Response of splenocytes to mitomycin-C-killed tumor cells was examined ex vivo using tumor cell restimulation assays. IFNγ production in response to stimulation was assessed through ELISA or FACS analysis after a 72-h incubation period (Untr. n = 10, Macs + DMSO n = 8, Macs + 13-cRA n = 10). h, Tumor growth expressed as a percentage of the initial volume. Mice were injected intravenously with LGP-Macs or Olfr644^−/− Macs pretreated for 4 h with DMSO or 13-cRA. i,j, FACS analysis: percentages of cells gated on GFP⁺F4/80⁺CD11b⁺ cells (LGP n = 6, LGP+13-cRA n = 6, OLFR644 n = 8, OLFR644 + 13-cRA n = 6) (i); and percentages of CD39⁺ cells gated on CD8⁺ T cells and T_reg cells gated on CD4⁺ T cells (LGP n = 6, LGP + 13-cRA n = 6, OLFR644 n = 8, OLFR644 + 13-cRA n = 6) (j). Statistical analyses were performed using two-tailed unpaired Student’s t-test. Values are presented as the mean ± s.e.m. All replicates represent biological replicates.