Fig. 6: Analysis of OR51E2 on human macrophages.

a, Volcano plot showing differentially expressed genes in prostate cancer tissues compared to normal tissue from The Cancer Genome Atlas. Genes are colored according to their log₂FC value (blue, log₂FC ≤ −0.5; red, log₂FC ≥ 0.5). Data were analyzed using GEPIA2. b, Representative confocal immunofluorescence images and quantification of human prostate cancer tissues (patient 1) showing expression of OR51E2 (red) in CD68⁺ macrophages (green). Nuclei were counterstained with DAPI (blue). Images were acquired with an SP8-II confocal microscope (Leica). Scale bar, 10 µm; number of fields = 5. c, Representative confocal immunofluorescence images and quantification of OR52E2 expression in human Thp1 cells with and without genetic deletion of OR51E2. Each dot represents one cell. n ≥ 10. d, Bar graphs showing percentages of CD206⁺ and HLA-DR⁺ macrophages gated on CD68⁺ upon exposure to conditioned media from PC3 tumor cells, in the presence or absence of partial genetic deletion of OR51E2. Results are expressed as the FC of the CM-tr. over the Untr. group (CD206: Untr. n = 4, short interfering RNA (siRNA) n = 5, CM-tr. or siRNA + CM-tr. n = 6; HLA-DR: Untr. n = 3, siRNA n = 3, CM-tr. or siRNA + CM-tr. n = 3). Statistical analyses were performed using two-tailed unpaired Student’s t-test. Values are presented as the mean ± s.e.m.