Extended Data Fig. 9: TGF-β does not decrease Eomes expression in day 4.5 in vitro-activated CD8+ T cells.
From: IL-4 impairs the formation of skin-resident memory CD8+ T cells
(a) Mean fluorescence intensity of Eomes expression gared on CD8+CD3+ T cells (left), median fluorescence intensity of Eomes expression gated on CD8+CD3+ T cells (middle), and mean fluorescence intensity of Eomes expression gated on Eomes+CD8+CD3+ (right) in CD8+ T cells that were activated in vitro (1 mg/mL CD3 and CD28 antibody) and then cultured with or without TGF-β (10 ng/mL) and with or without IL-4 (10 ng/mL) for 3 days. Data are pooled from five independent experiments. Eomes mean fluorescence intensity (left), *p = 0.0056; **p = 0.0033; ***p = 0.0017; ****p = 0.0014; *****p = 0.0013. Eomes median fluorescence intensity (middle), *p = 0.0065; **p = 0.003; ***p = 0.0021; ****p = 0.002; *****p = 0.0019. Eomes mean fluorescence intensity in Eomes+CD8+CD3+ T cells (right) *p = 0.0111; **p = 0.0043; ***p = 0.0037; ****p = 0.0028; *****p = 0.0006, two-tailed paired t-test. Mean ± SEM. (b) RT-qPCR-determined expression of Eomes in CD8+ T cells activated and treated with cytokines as in a. Data are pooled from five independent experiments. *p = 0.0099; **p = 0.0032; ***p = 0.0023; ****p < 0.0001, two-tailed paired t-test. Mean ± SEM.
