Extended Data Fig. 5: Targeting of GNA11_A guide and MLPH_A guide. | Nature Immunology

Extended Data Fig. 5: Targeting of GNA11_A guide and MLPH_A guide.

From: DNA hypomethylation traits define human regulatory T cells in cutaneous tissue and identify their blood recirculating counterparts

Extended Data Fig. 5

a, Sanger sequencing of GNA11 locus in human CD4+ T cells after CRISPR-Cas9 targeting with guide GNA11_A (top), scrambled control (middle) and not-CRISPRed genomic DNA (bottom) of human T cells. Blue boxes indicate position of GNA11_A guide. Lower part, percentage of aberrant sequences (not matching to genomic control sample). Dashed blue line indicates position of GNA11_A guide. b, Sanger sequencing of MLPH locus in human CD4+ T cells after CRISPR-Cas9 targeting with guide MLPH_A (top), scrambled control (middle) and not-CRISPRed genomic DNA (bottom) of human T cells. Blue boxes indicate position of MLPH_A guide. Lower part, percentage of aberrant sequences (not matching to genomic control sample). Dashed blue line indicates position of MLPH_A guide.

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