Extended Data Fig. 8: Ablation of KCa3.1 R350 methylation shows similar ATAC-Seq signature as of T cells activated in reduced Met.
From: Early methionine availability attenuates T cell exhaustion
a, PCA analysis of ATAC-Seq of KCa3.1WT- and KCa3.1R350A- activated with 2.5 ng/ml SIINFEKL for 24 h. n = 3. b, Common DAR (p < 0.05, fold change>1.5) between ATAC-Seq of OT-I cells expressing KCa3.1WT or KCa3.1R350A, or OT-I cells initially activated in 0.1 mM or 0.03 mM Met as in (a) and Extended Data Fig. 2b. c, Over-representation analysis of the common DAR from (b) showing the top 8 gene sets associated with exhaustion and activation. d, e, Top 10 motifs analyzed by HOMER in ATAC-Seq DAR’s from 24 h activated OT-I CD8+ T cells expressing KCa3.1R350A vs KCa3.1WT (d), and from the common DAR’s from (b, e). Colour corresponds to the common exhaustion-associated motifs also described in the indicated studies (blue and purple).
