Fig. 6: Ablation of KCa3.1 R350 methylation promotes T cell exhaustion in tumors and infection models.
From: Early methionine availability attenuates T cell exhaustion
a, Schematic of experimental design to assess congenically distinct KCa3.1WT and KCa3.1R350A OT-I T cells mixed at a 1:1 ratio and transferred into B16-OVA tumor-bearing Rag1−/− mice, analyzed D12 after T cell transfer. b–e Representative plot and frequency of PD-1+Tim-3+ (b), histogram and quantification of TOX MFI (c), quantification of TCF1 MFI (d) and frequency of IFNγ+TNF+ (e) and among KCa3.1WT and KCa3.1R350A OT-I TIL at D12 after T cell transfer as in a (n = 7 mice per group). f,g, Representative plot (f) and frequency of TCF1+Tim-3− Texprog and TCF1−Tim-3+ Texterm (g) in KCa3.1WT and KCa3.1R350A OT-I TIL at D12 after T cell transfer as in a (n = 7 mice per group). h, Schematic of experimental design to assess congenically distinct KCa3.1WT and KCa3.1R350A Thy1.1 P14 T cells, mixed at a 1:1 ratio and transferred into WT mice infected with LCMV-Clone-13. Spleens were analyzed at D9 postinfection. i, Representative plot and frequency of TOX+ in KCa3.1WT and KCa3.1R350A P14 CD8+ T cells from spleens at D9 postinfection as in g (n = 4 mice per group). j,k, Representative plots (j) and frequency of PD-1+Tim-3+ (k) in KCa3.1WT and KCa3.1R350A P14 CD8+ T cells from spleens at D9 postinfection as in g (n = 4 mice per group). l,m, Frequency of TCF1+Tim-3− Texprog (l) and TCF1−Tim-3+ Texterm (m) in KCa3.1WT and KCa3.1R350A P14 CD8+ T cells from spleens at D9 postinfection as in g (n = 4 mice per group). Data are mean ± s.d. Paired two-tailed Student’s t-test (b–e, g, i–m). Illustrations in a and h created with BioRender.com.
