Extended Data Fig. 1: Methionine availability during TCR activation determines T cell fate.
From: Early methionine availability attenuates T cell exhaustion
a, Log2 fold change (FC) in serum Met levels post LCMV-C13 chronic infection (data from ref. 7). b, c, Tumour growth (b) and survival (c) of B16-OVA tumour-bearing WT mice treated with or without OT-I CD8+ T cells activated in 0.1 or 0.03 mM Met for 30 min before restoring Met to 0.1 mM for 24 h, as described in Fig. 1d (n = 5 mice/group). d, Schematic of experimental design (Fig. 1d) for generation of LCMV-GP33 specific T cells, which were then activated as shown before injection into B16-OVA tumour-bearing Rag1−/− mice. e, Schematic design for ATAC-Seq on OT-I T cells initially activated in 0.1 or 0.03 mM Met followed by restoration of Met to 0.1 mM for 24 h. Data are mean±s.d. 2-way ANOVA (b), and Mantel-Cox log rank test (c). Illustrations in d, and e were generated with Biorender.com.
