Extended Data Fig. 8: Flow cytometry uncovers the impact of Larp4-KO on differentiation and function of tumor-specific CD8+ T cell within TME.
From: LARP4-mediated hypertranslation drives T cell dysfunction in tumors
(a) Flow cytometry gating strategy to identify PD-1hi TIM-3+ TEX cells and PD-1Int CX3CR1+ TEFF-like cells within TME. Endogenous cells were shown. (b) Representative flow cytometry graphs showing the percentage of PD-1int CX3CR1+ TEFF-like cells in control and Larp4-KO C9OT-I T cells within TME. PD-1int CX3CR1+ TEFF-like cells were gated from PD-1+ TIM-3− C9OT-I T cells. (c) Bar plots showing the MFI of CD25 in control and Larp4-KO C9OT-I T cells within TME. The MFI values were normalized to control cells (n = 6). (d) Representative flow cytometry graphs and bar plots showing the percentage of IFN-γ+ cells in control and Larp4-KO C9OT-I T cells within TME (n = 6). (e) Representative flow cytometry graphs and bar plots showing the percentage of TNF+ cells in control and Larp4-KO C9OT-I T cells within TME (n = 6). (f) Representative flow cytometry graphs and bar plots showing the percentage of IL-2+ cells in control and Larp4-KO C9OT-I T cells within TME (n = 6). (g) Flow cytometry graphs showing the percentage of IFN-γ+ IL-2+ (left) and TNF+ IL-2+ (right) cells in control and Larp4-KO C9OT-I T cells within TME. n, numbers of mice (c-f). For statistical analysis, data in bar plots are presented as mean ± SEM and unpaired two-sided Student’s t-tests were performed for (b-f).
