Extended Data Fig. 3: Correlation of SCG2 with MDSCs, CTLs, or patient survival.

a, Kaplan–Meier survival curves comparing overall survival of patients stratified by high (red) versus low (blue) SCG2 expression in TCGA CESC, TCGA MESO, GSE65858, GSE54236, and GSE1993 datasets. Survival analyses for TCGA CESC and TCGA MESO datasets were performed using GEPIA2 online tool (http://gepia2.cancer-pku.cn/#index) with median SCG2 expression as the cutoff. Survival data and analyses for GSE65858, GSE54236, and GSE1993 were obtained from the Tumor Immune Dysfunction and Exclusion (TIDE) database (http://tide.dfci.harvard.edu/), using optimal cut-off values provided by TIDE. Statistical significance was determined using the log-rank (Mantel–Cox) test. b-c, Correlation analyses between SCG2 expression and infiltration of myeloid-derived suppressor cell (MDSC) or cytotoxic T lymphocyte (CTL) across multiple tumor datasets. RNA-seq data were obtained from GEO and TCGA. Gene expression values in each dataset were log2-transformed and normalized using the sample average for TIDE analysis. Cytotoxic T lymphocyte (CTL) infiltration scores were calculated based on the average expression of CD8A, CD8B, GZMA, GZMB, and PRF1 within each dataset. Pearson correlation analyses were conducted between SCG2 and MDSC infiltration, as well as between SCG2 and CTL infiltration. Scatter plots for these correlations were generated using the ggplot2 package in R.