Extended Data Fig. 5: SCG2-LILRB4 interaction promotes tumor growth.
From: Secretogranin 2 binds LILRB4 resulting in immunosuppression
a, Immunoblot of SCG2 expression in MC38, B16F10 and LLC cells infected with lentiviruses encoding pLVX-SCG2-puro or empty vector (pLVX-puro). b, Tumor weights from WT or LILRB4-Tg mice subcutaneously injected with indicated SCG2-expressing or vector control MC38 (n = 7 or 9), B16F10 (n = 6 or 8), or LLC (n = 7) cells. Tumors were harvested and weighed at the endpoint. c, Tumor growth curve and tumor weights in LILRB4-Tg mice and WT mice injected subcutaneously with WT MC38 cells or mouse SCG2-expressing MC38 cells (n = 7 or 9). d, Tumor weights of MC38 tumor-bearing LILRB4-Tg mice intratumorally injected with recombinant SCG2 or PBS (n = 7). e, Tumor weights and representative images from MC38-Vec and MC38-SCG2 tumors in LILRB4-Tg mice intraperitoneally injected with anti-LILRB4 antibody or control IgG (n = 8). f, Flow cytometric analysis of LILRB4 expression on tumor-infiltrating M-MDSCs and G-MDSCs from tumor-bearing mice treated with anti-LILRB4 antibody (n = 8). g, Frequency of CD4+ and CD8+ T cells in peripheral blood of mice treated intraperitoneally with anti-CD4/CD8 antibodies or control IgG. h, Tumor weights and representative images from MC38-Vec and MC38-SCG2 tumor-bearing mice treated with anti-CD4/CD8 antibodies or control IgG (n = 6 or 8). i, Percentages of peripheral blood M-MDSCs, G-MDSCs, and macrophages after intraperitoneal treatment with anti-Ly6G or anti-CSF1R antibody (n = 5). j, Tumor weights from MC38 tumor-bearing mice treated with anti-Ly6G or anti-CSF1R antibodies (n = 5). k, Immune cell infiltration analysis of B16F10-Vec and B16F10-SCG2 tumors from WT and LILRB4-Tg mice (n = 7). l, Frequency of GZMB+ cells among tumor-infiltrating CD8+ T cells in MC38-Vec and MC38-SCG2 tumors (n = 9). m-n, Analysis of tumor-infiltrating immune cell populations in MC38-Vec and MC38-SCG2 tumors from mice treated with anti-LILRB4 antibody or control IgG (n = 7). Tumor growth curves are present as mean ± s.e.m.; other data are present as mean ± s.d. p values were determined by two-tailed unpaired Student’s t-test (d, i, and l), one-way ANOVA with Holm-Sidak’s multiple comparisons test (b, c (right), e, f, h, j, k, m, n), or two-way ANOVA with Tukey’s multiple comparisons test (c, left).
