Extended Data Fig. 3: SATB1 is essential for maintaining TPRO quiescence during chronic infection.
From: SATB1 is a key regulator of quiescence in stem-like CD8+ T cells
a-b, Representative flow plots and summary displaying gMFI of T-bet (a) and EOMES (b) in splenic sgCtrl (orange) and sgSatb1 (blue) P14 cells on day 21 p.i. with LCMV Cl13 (n = 5). c, Representative flow plots and summary of CD8+ T cells subset distribution in lymph nodes, showing TPRO (Ly108+ CX3CR1−), TEFF (Ly108− CX3CR1+), and TEXH (Ly108− CX3CR1−) sgCtrl (orange) and sgSatb1(blue) P14 cells on day 21 p.i. (n = 9). d, Representative flow plot and summary of TPRO1 (Ly108+ CD62L+) and TPRO2 (Ly108+ CD62L−) frequencies of sgCtrl and sgSatb1 P14 cells in lymph nodes on day 21 p.i. (n = 9). e, PCA plot of RNA-seq data for sgCtrl and sgSatb1 P14 cells on day 21 p.i. with LCMV Cl13 (n = 5). f, Heatmaps of 1767 DEGs between sgCtrl (838 genes) and sgSatb1 (929 genes) P14 cells (adjusted p value < 0.05); selected DEGs are indicated. g, GSEA of hematopoietic cell lineage commitment from KEGG (Pathway ID: mmu04640) between sgCtrl and sgSatb1 P14 cells. h-m, Flow and summary plots corresponding to Fig. 3j. Representative flow plot and summary of Ki67 expression (h), Annexin-V (i), granzyme B (j), TCF1 (k), LEF1 (l), and CD62L (m), and in sgCtrl and sgSat1 splenic P14 cells on day 14 post-transfer (n = 5). All data points (n) represent individual mice as biological replicates. Exact P-values are shown in each graph. Data in a-d and h-m are representative of 2-3 independent experiments. Two-sided paired t-test is used in a-b, h-j; two-sided multiple pair t-test with Holm-Å Ãdák correction was used in c-d; ns = not significant.
