Extended Data Fig. 4: SATB1 limits terminal differentiation in the chronic phase of infection.
From: SATB1 is a key regulator of quiescence in stem-like CD8+ T cells
a-d, Representative flow plots and summary showing gMFI of CD101 (a), LAG3 (b), TIM-3 (c), and TOX (d) in sgCtrl (control; orange) and sgSatb1 (SATB1-deficient; blue) splenic P14 cells on day 21 p.i. with LCMV Cl13 (n = 5). e, Heatmaps of DEGs representing inhibitory molecules between sgCtrl and sgSatb1 (adjusted p value < 0.05; n = 5). f-j, Flow and summary plots corresponding to Fig. 4h (n = 5). f, Summary of splenic P14 frequencies in sgCtrl (orange) and sgSatb1 (blue) as shown Fig. 4h. g, Representative flow plots and summary of Ki67+ percentages in P14 cells. h, Summary of absolute cell number in TPRO, TEFF, and TEXH subsets. i-j, Representative flow plots and summary LEF1+ percentages (i), and CD62L+ percentages (j) in P14 cells (n = 5). k, Summary data of viral titer in the sera from experimental mice receiving sgCtrl or sgSatb1 P14 cells (n = 5; bar and interval represent means ± s.d.). All data points (n) represent individual mice as biological replicates. Exact P-values are shown in each graph. Data in a-d and f-j are representative of 2-3 independent experiments; two-sided paired t-test was used in a-g and i-j; two-sided multiple paired t-test with Holm-Å Ãdák correction was used in h; unpaired t-test with Welch’s correction was used in k; ns = not significant.
