Extended Data Fig. 5: Satb1fl/fl-CD8cre mice phenocopy CRISPR-mediated SATB1-deletion mice.
From: SATB1 is a key regulator of quiescence in stem-like CD8+ T cells
a, Schematic of the experimental design: Satb1 + /+ CD8Cre (WT) or Satb1fl/fl CD8Cre (KO) mice were infected with LCMV Cl13 and analyzed at day 21 p.i. b, Frequency of GP33-41-specific CD8+ T cells in spleen. c-d, Representative flow cytometry plots of GP33-41 tetramer staining (c) and CD8+ T-cell subset distribution. e, Quantification of TPRO and combined TEFF + TEXH subsets among GP33-41-specific CD8+ T cells. f-i, Representative flow plots and summary of SATB1 (f), TCF-1 (g), PD-1 (h), and CXCR6 (i) expression in WT and KO GP33-41-specific CD8+ T cells. Data represent two independent experiments with n = 5 mice per group. All data points (n) represent individual mice as biological replicates. Bar and interval in graphs represent means ± s.d. Exact P-values are shown in each graph. Statistical significance was determined by two-sided unpaired t-test with Welch’s correction in b, f-i; multiple unpaired t-test with Holm-Å Ãdák correction was used in e.
