Extended Data Fig. 6: SATB1 restrains effector differentiation during acute infection.

a, Knockout efficacy of SATB1 on day 35 p.i. with LCMV Armstrong: representative flow plots and a summary of SATB1 gMFI comparing sgCtrl (control; orange) and sgSatb1 (SATB1-deficient; blue) P14 cells (n = 5). b, Representative flow plots depicting sgCtrl (top panel; orange) and sgSatb1 (bottom panel; blue) KLRG1⁺ IL-7Rα⁺ P14 short-lived effector cells (SLECs) and KLRG1⁻ IL-7Rα⁺ memory precursor effector cells (MPECs) isolated from blood at various post-infection timepoints. c-d, Summary of sgCtrl and sgSatb1 P14 SLECs (c) and MPECs (d) at various post-infection timepoints shown in b (n = 12; data points with interval represents means ± s.d.). e, Representative flow plots displaying the frequencies of sgCtrl (orange) and sgSatb1 (blue) splenic P14 cells on day 7 p.i. with LCMV Armstrong. f, Representative flow plots and summary depicting the frequencies of sgCtrl and sgSatb1 SLECs and MPECs isolated from splenocytes (n = 5). g-i, Representative flow plots and summary showing the percentages of TCF-1⁺ (g), CXCR3⁺ (h), and CX₃CR1⁺ P14 cells in sgCtrl and sgSatb1 on day 7 p.i. (n = 5). All data points (n) represent individual mice as biological replicates. Exact P-values are shown in each graph. Data in a-i are representative of 2-3 independent experiments. Statistical significance was determined by two-side paired t-test in a and g-I; multiple paired t-test with Holm-Šídák correction was used in c-e.

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