Extended Data Fig. 7: SATB1 is necessary for differentiating TCM but not TRM cells.
From: SATB1 is a key regulator of quiescence in stem-like CD8+ T cells
a, Summary of absolute number of sgCtrl (control; orange) and sgSatb1 (SATB1-deficient; blue) P14 cells in spleen on day 35 p.i. with LCMV Armstrong (n = 4). b-c, Representative flow plot and summary showing the percentages of CX3CR1+ (b) and KLRG1+ (c) splenic P14 cells in sgCtrl and sgSat1 on day 35 p.i. (n = 5). d, Representative flow plots displaying the frequencies of sgCtrl and sgSatb1 P14 cells in lymph nodes on day 35 p.i. (n = 5). e, Representative flow plots and summary depicting sgCtrl and sgSatb1 CD62L+ central memory (TCM) and CD62L− effector memory (TEM) cells in lymph nodes on day 35 p.i. (n = 5). f, Representative flow plots and summary showing percentage of TCF-1+ TCM cells in lymph nodes of sgCtrl and sgSatb1. (n = 5). g, Representative flow plots displaying the frequencies of sgCtrl and sgSatb1 P14 cells in intestinal intraepithelial lymphocytes (IEL) on day 35 p.i. h, Representative flow plots and summary showing percentages of sgCtrl and sgSatb1 CD69+ CD103+ resident memory (TRM) cells from IEL on day 35 p.i. (n = 5). All data points (n) represent individual mice as biological replicates. Exact P-values are shown in each graph. Data in a-h are representative of 2-3 independent experiments. Statistical significance was determined by two-side paired t-test in a and a-b and h; multiple paired t-test with Holm-Å Ãdák correction was used in e. ns = not significant.
