Extended Data Fig. 8: SATB1 regulates the epigenetic program of stemness in TPRO cells.
From: SATB1 is a key regulator of quiescence in stem-like CD8+ T cells
P14 cells transduced with sgCtrl and sgSatb1 were adoptively transferred into recipient mice, followed by LCMV Cl13 infection. On day 21, p.i., these P14 cells were sorted and analyzed by ATAC-seq. Four replicates were included in each group. a, Representative flow plots showing sgCtrl or sgSatb1 P14 cells sorting panel (a) and post-sort purity (b). c, Spearman distance analysis using all differentially accessible peaks (DAPs) between indicated populations (FDR ≤ 0.01, lfc≥2). Color indicates distances. d-e, Sequencing tracks of SATB1 ChIP seq (GSE191146), ATAC of TPRO subset (GSE149752), and CUT&TAG of TPRO H3K27ac (GSE149810) at indicated (d) Il7r locus and (e) Tcf7 locus. The differentially accessible peaks between sgCtrl and sgSatb1, illustrated in Fig. 6j-k, are highlighted with a red bar.
