Extended Data Fig. 10: SATB1 plays a role in maintaining the chromatin architecture in TPRO-associated genes.
From: SATB1 is a key regulator of quiescence in stem-like CD8+ T cells
a, Whole-genome Hi-C contact matrices at 100 kb resolution for wild-type (WT left) and Satb1 knockout (Satb1-KO; right) CD8+ T cells. b, Saddle plots ranked by eigenvector percentiles display chromatin compartmentalization in WT (left), KO (middle) conditions, and the log2 ratio of KO/WT compartmentalization (right). c, a Venn diagram displaying differential and shared topologically associating domains (TADs) between WT and KO at 50 kb resolution. d, Pathway enrichment analysis of genes within WT-specific (top panel) and KO-specific TADs (bottom panel), identified using HOMER-based annotation and ShinyGO. e, Loop pileup of TPRO-specific enhancer sites (N = 2,151) anchored at genome regions in WT (right) and KO (left), visualized at 10 kb resolution. f, Loop pileup of enhancer regions associated with T-cell differentiation (N = 2,670) anchored at genome regions in WT (right) and KO (left), visualized at 10 kb resolution. g-h, Hi-C heatmap maps at 10 kb resolution displaying distance-normalized chromatin interactions in WT (top) and KO (bottom) at the Tcf7 (g) and Myb (h) loci, visualized on Pygenome. The green boxes mark chromatin interactions with changes. ATAC-seq tracks of sgCtrl (orange) and sgSatb1 (blue) CD8+ T cells are shown between Hi-C heatmaps.
