Extended Data Fig. 2: SATB1 deficiency promotes CD8⁺ T cell proliferation in various tissues in chronic viral infection.

a-b, Knock-out efficacy of SATB1 in LCMV Cl13 infection; representative flow plots (a) and western Blot (b) of SATB1 comparing sgCtrl (control; orange) and sgSatb1 (SATB1-deficient; blue) P14 cells. c-d, Summary of liver P14 frequency (c) and numbers (d) at various timepoints p.i. (n = 5). e-f, Summary of lung P14 frequency (e) and numbers (f) at various timepoints p.i. (n = 5). g, Experimental design of CRISPR-RNP in single-transfer experiment: CD45.1⁺ P14 CD8⁺ cells were transduced with sgCtrl (control; orange cell) or sgSatb1 (SATB1-deficient; blue cell) and then adoptively single-transferred into separate CD45.2⁺ C57BL/6 recipient mice, which were subsequently infected with LCMV Cl13. h, Flow plot and summary showing the frequency of splenic sgCtrl and sgSatb1P14 cells in single-transfer experiment on day 21 p.i. (n = 5). i, Representative flow plots and summary displaying the frequencies of sgCtrl and sgSatb1 P14 cells in lymph nodes on day 8 p.i. j, Representative flow plots and summary data of SATB1 protein expression in Ly108⁺ precursor and Tim3⁺ early effector cells on day 8 p.i. (n = 5). Data in a-j are representative of 2-3 independent experiments, and data points with bars in c-f are means ± s.d. All data points (n) represent individual mice as biological replicates. Exact P-values are shown in each graph; ns = not significant; two-sided multiple pair t- test with Holm-Šídák correction was used in c-f, and i; two-sided unpaired t-test was used in h; two-sided paired t-test was used in j.

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