Extended Data Fig. 6: Analysis of tumor-infiltrating T cells.

a, Upper panels: The abundance of CD8⁺ T cells in primary KPC tumors on the day of tumor resection (Day 14) determined by flow cytometry. Analyses of total CD8⁺ T cells as well as CD69 and CD44 expression were conducted to determine early and late activation states of CD8⁺ T cells, respectively. The abundance of total and activated CD8⁺ T cells is presented as a % fraction of total CD3⁺ T cells. For each data point, three tumors were pooled and dissociated to isolate lymphocytes for FACS analysis. Total 6-9 tumors were examined. Lower panels: The number of total CD8⁺ T cells or activated CD8⁺ T cells per tumor was calculated from the total live cell count after tumor dissociation and the % fractions of these T cells as determined by flow cytometry (Fig. 4a). For each data point, three tumors were pooled, and the obtained cell number was divided by three to yield average T cell count/tumor. Total 6-9 tumors were examined. b, A similar analysis of tumor-draining CD4⁺ T cells. A representative result of two independent experiments is shown for each analysis. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant.